![]() ![]() Components identification was performed using GC-MS analysis. Data were analyzed by probit analysis to obtain IC 50 values. Cytotoxic test on T47D cell line was perform for extracts of each separation stage. ![]() The active compound was separated using column chromatography. The extracts were tested their cytotoxic activity on T47D cell line using MTT method. Petroleum ether, chloroform, ethyl acetate, and ethanol were used to extract N. This study conducted to examine the cytotoxic activity of Nigella sativa seeds and identify its components on T47D breast cancer cells. Nigella sativa is one of the plants used as anticancer empirically. Therefore, alternative cancer treatment by using plants as traditional medicine was expected to reduce side effects. Cancer treatment with surgery, chemotherapy, and radiology often cause undesirable side effects. The results suggested that NS extracts have the activity to reduce the proliferation of cardiac cells in DMBA induce female mice.īreast cancer is one of the main causes of death in women. ![]() The AgNOR results indicated the presence of the inhibitory activity of NSS on cardiac cell proliferation in the treated groups. ![]() H&E staining results showed that there was a significant difference in cardiac histopathology pro le between the extract treatment group, DMBA group, and corn oil group. The chloroform extract of NS seeds administer with 3 doses, namely 250, 500, and 750 mg/kg administered weekly and during exposure to DMBA. DMBA was administered twice a week for five weeks. Sprague Dawley rats (SD) used in this study are divided into five groups. This research conducted to study the anticarcinogenesis effects of N.sativa extract (NS) seed on 7.12-dimethylbenz anthracene-induced female mice. Nigella sativa has anti-cancer activity in vitro and in vivo. DMBA has been widely used in various models of cancer research, because it can induce most of the tumors, including proliferation in the cardiac cell. DMBA is Polycyclic Aromatic Hydrocarbons carcinogenic compounds (PAHs), activated by cytochrome P450 enzyme, and react with DNA to initiate cancer. 7.12-dimethylbenz anthracene (DMBA) is one of the most potential carcinogenic agent. ![]()
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